ISHS Acta Horticulturae 175: Symposium on Physiology of Productivity of Subtropical and Tropical Tree Fruits
PLANT REGENERATION VIA SOMATIC EMBRYOGENESIS IN CALLUS CULTURE OF LOQUAT |
| Authors: | W. Ho, C. Chang, S. Huang |
| Abstract:
Techniques involving in the formation of loquat (Eriobotrya japonica Lindl.) plantlet via somatic embryogenesis from callus cultures were described. Shoot tips and immature embryos from seed were used as initial explants for embryogenic callus induction. The embryogenic callus was derived from shoot tips and immature embryos cultured on Murashige & Skoog (1962) agar medium supplemented with thiamine 1 mg/1, 2,4-D 0.5–2.0 mg/1 and BA 0.05–1.0 mg/1. Shoot tips sized 1–2 mm dissected from late fall or winter buds were more suitable for embryogenic callus induction than those from other seasons. Immature embryos, 1–7 mm long, were all responsible for embryogenic callus formation, whereas 2–4 mm young embryos were more successful in forming embryogenic callus. The differentiation of somatic embryo had occurred on the surface of embryogenic callus. Various stages of embryoid development including globular, heart-shaped and torpedo-shaped embryoids were observed through morphological and anatomical studies. The fully developed embryoid germinated on MS medium (1–2 mg/1 BA and 5% coconut milk) contained both cotyledons and shoot-root axis. The formation of root from germinating embryoids was enhanced by supplementing the medium with 1.0 mg/1 NAA. Secondary embryogenic callus and embryoids were formed when embryogenic callus and embryoids were subcultured on the embryogenic callus induction medium. | |
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