ISHS Acta Horticulturae 169: International Workshop on Improvement of Sweet and Sour Cherry Varieties and Rootstocks
MICROPROPAGATION OF SOUR CHERRY, CULTIVAR SCHATTENMORELLE |
| Author: | B. Borkowska |
| Abstract:
Primary explants of actively growing tips of sour cherry, Schattenmorelle cv. were established and subsequently proliferated on the salt and microelements mixture of Murashige-Skoog, supplemented with 0.1 mg NAA, 1 mg BA and 0.1 mg GA3 (per liter). The pH was adjusted to 5.2 – 5.3 Half-strength MS salts and full-strength microelements supplemented with the ammoinium salt of IBA were used as the rooting medium. After 4 – 5 weeks the root system was completely formed and the plants were moved to pots with a peat - pine bark - sand (3:1:1) mixture. First 5 weeks of multiplication phase, were characterized by rapid increase of the weight of cultures, high rate of proliferation, increase of invertase activity and level of sugars. Chlorophyll content at this time was more or less constant. Weeks 5 to 7th were characterized by stagnation of all processes and over 7th weeks by senescence of cultures. In our conditions 5 weeks was the optimal culture period for multiplication. Its prolongation for 6 – 7 weeks allowed to obtain longshoots used for rooting. | |
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