ISHS Acta Horticulturae 169: International Workshop on Improvement of Sweet and Sour Cherry Varieties and Rootstocks
MULTIPLICATION CONFORME DE SUJETS PORTE-GREFFE ET DE VARIETES DE CERISIERS PAR LA CULTURE IN VITRO |
| Author: | Ph. Druart |
| Abstract:
True-to-type propagation of rootstocks and varieties of cherry trees by in vitro culture The micropropagation technique from meristem culture, applied to more than 200 different clones of fruit trees in our research station, is described. Commercial propagation planning must take in account the variation of propagation rate on the subcultures, the troubles of vitrification during the multiplication cycle and apex necrosis specialy at the end of elongation stage and in the rooting stage. In vitro rooting rate and quality are subjected to the culture conditions before and during the rooting stage. Shoot elongation on tufts arising from axillary branching, increases the number of roots per plantlet as darkness stay (9 days) at the beginning of the rooting stage and proline in the rooting medium. Riboflavin reduces the callus and improves the root elongation. Acclimatized plantlets must be cold treated before transplanting to the nursery. Propagation by cuttings from acclimatizing plantlets seemed very easy. The development of lateral rooting in the rooting stage and root hair in cold room at 2°C before acclimatization was observed. | |
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