ISHS Acta Horticulturae 169: International Workshop on Improvement of Sweet and Sour Cherry Varieties and Rootstocks
DETERMINATION OF PEROXIDASES AND ACID PHOSPHATASES IN THE PHLOEM OF CHERRY GRAFTS BY MICROSCOPIC PHOTOMETRY |
| Authors: | W. Feucht, P.P.S. Schmid, E. Christ |
| Abstract:
Quantitative determinations of peroxidases and acid phosphatases from the phloem of Prunus grafts were performed by microscopic photometry. The recently grafted scions of 'Sam' (P. avium L.) on Cer W clones (P. cerasus L.) were harvested 1, 3 and 8 weeks after splice grafting and transversal cuts were made each 4 cm below and above the union. Acid phosphatases approached maximum levels after the 1 st week and then declined progressively to the 8th week. Most responsive were cambium and younger phloem followed by mature phloem, whereas phloem rays stained less. Cells forming oxalate crystals stained in the first week. Generally, more phosphatases were in the scion than in the rootstock. Peroxidases were determined after week 3 and 8. At the first date the staining intensity was markedly higher as compared with the second. Most responsive were the mature phloem, followed by younger phloem and cambium. The ray walls reacted likewise strongly. Further, sieve plates were responsive to some extent. Especially during week 3 the peroxidases were more pronounced above the union. | |
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