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ISHS Acta Horticulturae 168: II Symposium on Winter Hardiness in Woody Perennials

ATPASES IN RELATION TO FREEZING INJURIES IN PURIFIED PLASMA MEMBRANE VESICLES FROM NONACCLIMATED SEEDLINGS OF PINUS SYLVESTRIS

Authors:   J. Hellergren, S. Widell, T. Lundborg
Abstract:
The activities of a K,Mg-ATPase from purified plasma membrane vesicles from nonacclimated needles of Pinus sylvestris have been studied in relation to different freezing temperatures. As a comparison the enzyme cytochrome c oxidase was also studied.

After freezing to lethal temperatures, the activity of the K,Mg-ATPase decreased to zero, while that of cytochrome c oxidase did not significantely change. This indicates the importance of the K,Mg-ATPases as the primary target of freezing injuries.

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