ISHS Acta Horticulturae 167: II Symposium on Growth Regulators in Floriculture
INVERTASE INHIBITOR - CONTROL OF SUCROSE TRANSPORTATION FROM PETALS TO OTHER FLOWER PARTS |
| Authors: | J. Halaba, R. N. Rudnicki |
| Abstract:
It has been well documented that invertase inhibitor is synthetised in carnation petals during their senescence (Halaba and Rudnicki, 1983). It seems that decrease of the invertase activity about 2 days before wilting is closely accompanied by movement of sucrose out of the petals into neighboring organs (Nichols and Ho, 1975). This process of 14C-sucrose movement was investigated by Nichols and Ho (1975). The aim of this experiment was to determine the influence of the invertase inhibitor activity on the sucrose transportation from carnation petals, during their senescence, to the receptacle and ovary. Petals of carnation flowers, (Dianthus caryophyllus L.) cv. White Sim were fed with 14C-sucrose, with or without cycloheximide for 4 hours. IAA in a lanolin paste was applied on the stigma. Activity of invertase and invertase inhibitor were investigated in petals. Invertase activity decreased very rapidly in senescing petals. The cycloheximide treatment prevented the decrease of invertase activity, and completely blocked synthesis of invertase inhibitor. Radioassays of plant material showed that cycloheximide treatments decreased the transport of radioactive substances from petals to the receptacle and ovary. Receptacles and ovaries from flowers not treated with cycloheximide contained 7 to 10 times more radioactivity than those treated with cycloheximide. It is supposed that during senescence, the invertase inhibitor breaks sucrose hydrolysis to glucose and fructose and thereby enables carbohydrates to transfer from wilting petals to neighboring organs. | |
Download Adobe Acrobat Reader (free software to read PDF files) | |
