ISHS Acta Horticulturae 166: Propagation of Ornamental Plants
SOME ASPECTS OF STOCK-PLANT PREPARATION FOR TISSUE CULTURE PROPAGATION |
| Authors: | P. Debergh, L. Maene |
| Abstract:
Growing stock plants for tissue culture under hygienic conditions can help a lot to overcome contamination problems during the initiation of the cultures. Appropriated planting of the stock plants allow the development of more suited primary explants. The environmental conditions during the stock-plant preparation help to standardize the explants and to minimize browning problems. Juvenility can be induced in the stock plants by spraying or injecting growth substances or by grafting on seedlings. The use of antibiotics is not efficient to cure the stock-plants from endogenous contaminations. Reactions in vitro can be controlled by the fertilization of the stock-plants in the greenhouse, or by an hormonal pretreatment. | |
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