ISHS Acta Horticulturae 164: VI International Symposium on Virus Diseases of Ornamental Plants
SOLUBILITY OF CHRYSANTHEMUM CHLOROTIC MOTTLE VIROID IN LICL SOLUTIONS |
| Authors: | S.O. Kawamoto, R.K. Horst, S.-M. Wong |
| Abstract:
The 2M LiCl insoluble RNA extracts of both the severe and latent strains of chrysanthemum chlorotic mottle viroid (ChCMV-s and CHCMV-l) have higher infectivity titers than do the soluble fractions. Chrysanthemum stunt viroid, potato spindle tuber viroid, citrus exocortis viroid, and columnea viroid are primarily soluble in 2M LiCl. ChCMV-s and ChCMV-l migrate at the same rate as the 7s RNA in 5% polyacrylamide gels. No RNA band unique to ChCMV-infected tissue was observed in 5, 10, or 15% non-denaturing gels. Attempts to recover infectious ChCMV RNA from gels under fully denaturing conditions were unsuccessful. | |
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