ISHS Acta Horticulturae 164: VI International Symposium on Virus Diseases of Ornamental Plants
RATES OF REINFECTION WITH VIRUS IN COMMERCIAL CARNATION, CHRYSANTHEMUM AND GLADIOLUS CROPS |
| Authors: | Jane R. Moran, Jenny M. Wilson |
| Abstract:
Surveys were conducted on commercial carnation crops for infection with carnation mottle virus (CMoV), and on commercial chrysanthemum crops for infection with chrysanthemum virus B (CVB), tomato aspermy virus (TAV), and tomato spotted wilt virus (TSWV). The plants tested were derived from ordinary commercial stocks or derived from a Plant Improvement Program (PIP) operated by the Victorian Department of Agriculture. On properties only growing PIP carnations infection with CMoV averaged 0.95% and on properties growing ordinary commercial stocks infection averaged 100%, PIP carnations grown in conjunction with ordinary stocks had an average incidence of CMoV of 19.84%. A system of pooling samples into different sized batches and sequential testing of batches was used to conserve labour. All samples were tested using the enzyme-linked immunosorbent assay (ELISA) with a urease-antibody conjugate. Ordinary commercial stocks of chrysanthemums were often grown in close proximity to PIP chrysanthemums but re-infection was low. Infection with CVB and TAV averaged 9% and 7% in PIP carnations and 81% (CVB) and 23% (TAV) in ordinary commercial stocks. Tomato spotted wilt virus was only found in one plant. All chrysanthemums were tested by mechanical inoculation to indicator plants. Field trials on a commercial gladiolus grower's property showed that virus tested gladiolus grown 0.5 and 2 km from infected crops did not become infected with either bean yellow mosaic virus (BYMV) or cucumber mosaic virus (CMV). Virus tested plants grown amongst infected gladiolus averaged 16.5% infection with BYMV and 0.002% infection with CMV after one season. All tests for virus were done using ELISA. | |
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