ISHS Acta Horticulturae 164: VI International Symposium on Virus Diseases of Ornamental Plants
IN VITRO MANIPULATION OF VIRUS CONCENTRATIONS IN HYBRID LILIES |
| Authors: | D. Cohen, K.S. Milne, M.J. Hyland |
| Abstract:
The ELISA serological technique was used to monitor lily symptomless virus (LSV) and tulip breaking virus (TBV) levels in in vitro grown bulblets and newly-formed adventitious bulblets arising from bulb scales of Lilium x parkmanii hybrids. At 30C, both viruses were reduced to low levels in bulb scales and were almost undetectable in new bulblets. Levels of both viruses were highest at 15C and below. Virazole (10 mg/l) markedly reduced LSV titre, but was ineffective against TBV. Following transfer of bulblets with low LSV titre from 30C to 20C, titre remained low for one to three weeks before a sharp rise was detected. The significance of these results is discussed in relation to both the production of high health lilies and the reliable indexing of plants following attempted therapy. | |
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