ISHS Acta Horticulturae 164: VI International Symposium on Virus Diseases of Ornamental Plants
LIGHT MICROSCOPIC TECHNIQUES FOR DETECTING ORCHID VIRUSES |
| Authors: | N.-J. Ko, F.W. Zettler, J.R. Edwardson, R.G. Christie |
| Abstract:
Light microscopy was investigated as a tool for diagnosing orchid viruses. Inclusions induced in orchid tissues by odontoglossum ringspot virus, cymbidium mosaic virus, cucumber mosaic virus, two bacilliform viruses, and a potyvirus from Masdevallia were detected by this method. These studies were confirmed by electron microscopy, immunofluorescence microscopy, and immunodiffusion serology. Light microscopy was reliable for diagnosing these viruses, and was a useful alternative in situations when other techniques (bioassay, serology, or electron microscopy) could not be used. | |
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