ISHS Acta Horticulturae 164: VI International Symposium on Virus Diseases of Ornamental Plants
USE OF ANTISERA TO CYLINDRICAL INCLUSION BODIES OF POTYVIRUSES FOR VIRUS SCREENING AND IDENTIFICATION |
| Authors: | J. Hammond, R.H. Lawson |
| Abstract:
Cylindrical inclusion proteins of bean yellow mosaic virus (BYMV), iris mild mosaic virus (IMMV) and iris severe mosaic virus (ISMV) can be detected and differentiated by polyacrylamide gel electrophoresis from relatively small samples of infected plant material. Inclusion proteins of IMMV and ISMV recovered from acrylamide gels, and of BYMV prepared from larger scale extractions, have been used as immunogens in mice with the aim of preparing monoclonal antibodies for use in virus screening. Rabbit antiserum to the inclusion proteins of BYMV can be used in indirect ELISA to detect infection reliably in pea and Nicotiana benthamiana, but less reliably in gladiolus. Detection of the inclusions from gladiolus by indirect ELISA was improved when a fluorescent substrate was used instead of a chromogenic substrate, but with less sensitivity than for coat protein. Fluorescent ELISA was also found to increase the sensitivity of indirect ELISA for cucumber mosaic virus and BYMV coat proteins. | |
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