ISHS Acta Horticulturae 164: VI International Symposium on Virus Diseases of Ornamental Plants
ON SEROLOGICAL DETECTION OF MYCOPLASMS IN PLANTS |
| Authors: | W. Welvaert, G. Samyn |
| Abstract:
Due to the impossibility of growing mycoplasm isolates from plants with phyllody symptoms on artificial media, the production of antisera to such isolates is very difficult. Some methods, however, are described to obtain antisera from extracts of infected plants or insects which can be used for further diagnosis. The specificity that such techniques can offer is especially valuable when compared with other staining methods. The metabolism-inhibition test as well as the growth-inhibition test was used to determine the relationship of MLO-isolates on Vinca rosea with respect to Spiroplasma citri and Acholeplasma laidlawii. There was only a partial relationship between Spiroplasma citri and the Daucus isolate which was confirmed with FITC-immunofluorescence. | |
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