ISHS Acta Horticulturae 164: VI International Symposium on Virus Diseases of Ornamental Plants
RAPID IN VITRO PROPAGATION OF VIRUS-INDEXED GLADIOLI |
| Authors: | A.E. Logan, F.W. Zettler |
| Abstract:
An efficient tissue-culture system with the potential of producing more than 50 000 virus-free gladiolus (Gladiolus x hortulanus) transplants within 30 weeks from a single shoot-tip derivative was developed. Apical shoot-tips (0.5–0.7 mm) were established (Stage I) on an agar medium containing Murashige-Skoog salts, 1.0 mg/l kinetin, and 0.1 mg/l naphthylacetic acid. For shoot proliferation (Stage II), plantlets were transferred 7–8 weeks later to a similar medium without auxin. Plantlets were subcultured on Stage II medium every 3–4 weeks as needed and yielded 11–15 axillary shoots per subculture for all 23 gladiolus cultivars. For root initiation (Stage III), plantlets were transferred to a medium containing ½-strength Murashige-Skoog salts, 1.0 mg/l naphthylacetic acid, 0.5% powdered charcoal, and a substrate consisting of horticultural grade vermiculite instead of agar. Roots developed readily within 4–5 weeks on this medium, after which plants could be transferred directly to soil with very high explant survival rates. Plantlets were indexed for cucumber mosaic and bean yellow mosaic virus by double radial immunodiffusion serology, electron microscopy and/or by a modified enzyme-linked immunosorbent assay method. None of the original 48 apical shoot-tip derivatives was infected with either virus, whereas 15 of 17 explants derived from axillary buds (0.3–0.5 cm) were infected. | |
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