ISHS Acta Horticulturae 150: International Symposium on Substrates in Horticulture other than Soils In Situ
USE OF RECIRCULATING HYDROPONIC SYSTEMS FOR MACRO- AND MICROPROPAGATION STUDIES. |
| Authors: | Paul E. Read, S. Garton, K. Louis |
| Abstract:
Recirculating hydroponic systems, modifications of Cooper's "nutrient film system" (NFT), have been successfully employed to grow stock plants for various macro- and micropropagation studies. The influence of nitrogen levels on in vitro performance of tomato leaf explants was investigated and N, P and K effects on cutting production, rootability of cuttings and shoot production in vitro of willow (Salix spp.) stock plants were also studied. Willow cutting number was enhanced by the highest N-P-K level, but rooting of cuttings and number of shoots produced in vitro were best with the medial level. Successful inoculation of alder (Alnus) roots with the symbiotic N-fixing actinomycete, Frankia spp. was achieved, thus enabling propagation and Salix-Alnus interplanting research to be conducted. These species and several others have been held in NFT, in some cases up to 2 years, with continuous function as stock plants for macro- and/or micropropagation. | |
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