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ISHS Acta Horticulturae 147: Symposium on Production Planning in Glasshouse Floriculture

RAPID VEGETATIVE PROPAGATION OF EUCHARIS GRANDIFLORA IN VITRO

Authors:   R.L.M. Pierik, P.A. Sprenkels, J. van Bragt
Abstract:
To increase the propagation rate and to produce homogeneous plant material for flower production, a simple in vitro system was developed to clone Eucharis grandiflora. Excised bulb scale segments produced adventitious bulblets on the basal sides. Factors affecting formation and growth of these bulblets are described.

Nine weeks after excision of the scales the then formed bulblets were subcultured in vitro to study rooting, bulb growth and leaf formation in the following 9 weeks. The complete plants were then transferred to soil to study further growth.

First flowering of the in vitro produced plants was observed 16 months after the start of the in vitro culture. The in vitro progeny was homogeneous and the plants, including the flowers, were not visibly different from the original genotype.

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