ISHS Acta Horticulturae 134: Regulation of Growth and Endogenous Hormones, XXI IHC
QUANTIFICATION OF SOLUBLE ETHYLENE IN PLANT TISSUE |
| Authors: | G.W. Apel, M.E. Patterson |
| Abstract:
Ethylene in the gaseous phase is readily quantified but reveals little about the physiological level in solution. Ethylene in solution has been measured directly by bubbling inert gas through the solution stripping it of its dissolved gases thence through a cryogenic trap at -95°C to collect the ethylene. Two rotary sampling valves allow the trap to be isolated, heated to 100°C, and flushed with carrier gas which introduces the ethylene sample directly into a gas chromatograph. Solution volumes of 0.5 cm3 can be conveniently introduced for stripping and quantification of dissolved ethlene. Values for Henry's Law constant for ethylene in water obtained by this method compare favorably with published values. Concentrations of ethylene in solution in postclimacteric apples ranged from 1 to 24 ng cm-3 and correlated well with internal gas concentration (R2=0.96). Apparent Henry's Law constants for individual fruits were consistent and did not differ between cultivars. | |
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