ISHS Acta Horticulturae 131: In Vitro Culture, XXI IHC
IN VITRO PROPAGATION OF APPLE ROOTSTOCK - EMLA 25 |
| Authors: | G.S. Cheema, D.P. Sharma |
| Abstract:
Propagation of shoot-tips and axillary buds of apple rootstocks-EMLA-25 was obtained on modified Murashige and Skoog (MS) medium containing benzyladenine (BA) and naphthalene-acetic acid (NAA). Subculturing the shoots on MS medium supplemented with BA (1 mg/l) and 0.2 mg/l indolebutyric acid (IBA) resulted in 25–30 fold multiplication every 4 weeks from a single shoot tip or axillary bud, with best shoot extension and leaf growth. Both these media favoured the development of adventitious buds in isolated internodes and internodes of the development shoots and their basal calli. One hundred percent rooting of isolated individual shoots was achieved in 3 weeks on a cytokinin-free modified MS medium supplemented with 2 mg/l IBA and 2% activated charcoal. Phloroglucinol (162 mg/l) showed deleterious effect on cultures when supplemented in the culture medium. The rooted plantlets could be successfully transplanted to soil. The basic and applied aspects of results of the research accomplished are discussed in the light of relevant literature reviewed. | |
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