ISHS Acta Horticulturae 131: In Vitro Culture, XXI IHC
IN VITRO PROPAGATION OF PISTACIA SPECIES |
| Authors: | M. Barghchi, P.G. Alderson |
| Abstract:
Procedures have been developed for the clonal propagation of Pistacia vera L. from aseptically germinated seedlings and seed raised plants up to two years old. Proliferating shoot cultures were established from shoot tip and nodal bud explants of P. vera L. and the commercial varieties 'Ohadi' and 'Kalleghochi' cultured on Murashige and Skoog (MS) medium supplemented with benzylaminopurine (4 mg l-1). Adventitious (epiphyllous) shoots were produced on leaves of cultured shoots which contacted the culture medium. Cultured shoots of P. vera L. were rooted in vitro using MS medium (half strength macronutrients) containing 2.5 mg l-1 indolebutyric acid for root initiation, followed by subculture onto hormone-free medium for root development. Rooted shoots were readily established in peat based compost. Callus cultures of P. vera were established from shoot tips and nodal tissues cultured on MS medium containing auxin and cytokinin, and maintained by subculturing onto fresh medium. Extending the time interval to two months or longer between subcultures resulted in changes in the morphology of the callus and the production of some shoots in approximately 10% of the cultures. | |
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