ISHS Acta Horticulturae 131: In Vitro Culture, XXI IHC
EFFORTS IN SOMATIC EMBRYOGENESIS OF PHASEOLUS VULGARIS L. |
| Authors: | A. Allavena, L. Rossetti |
| Abstract:
Although the concept of cell totipotency is widely proved, in vitro morphogenesis has not yet been achieved for a large number of cultivated species. In tissue and cell culture of beans, the factors controlling shoot morphogenesis and somatic embryogenesis are still unknown. The reported data suggest a possible way for future research. Two main series of experiments were carried out following a classical protocol:
Genotypes P 263, Taylor's H., two F1 hybrids both with P 263 as parent, other varieties and breeding lines were used in different experiments. Pieces of primary leaves from 8–10 day old seedlings aseptically grown or immature embryos excised 10 days after anthesis were used as explants. Primary and secondary cultures were maintained in diffuse light, 16 to 8 hours light-dark cycle at a temperature of 25±0.5°C: suspension cultures were shaken at 120 r.p.m. After 15–18 days embryo-like structures developed only from some suspension cultures. No full plants have been obtained. In most cases the cotyledonary domes of the embryo-like structures degenerated into roots; in other cases shoot buds of aging cultures degenerated into callus. The following considerations can be drawn:
| |
Download Adobe Acrobat Reader (free software to read PDF files) | |
