ISHS Acta Horticulturae 131: In Vitro Culture, XXI IHC
EFFECT OF STOCK PLANT NUTRITION ON MACRO- AND MICRO-PROPAGABILITY OF SALIX |
| Authors: | S. Garton, Paul E. Read, Rouse S. Farnham |
| Abstract:
Rooted cuttings of ten willow clones were grown hydroponically in three nutrient solutions differing in the concentrations of nitrogen, potassium and phosphorus. After 10 weeks in the greenhouse, cuttings were taken and placed in peat pellets under intermittent mist. Lateral buds from the soft subapical portions of actively growing shoots were aseptically transferred to a medium designed to promote shoot proliferation in the explants. Organogenesis in macro and micropropagules was profoundly influenced by the clonal origin or genotype of the plant material. The effects of the stock plant nutrition treatments were less pronounced than the clonal effects in macropropagation. Cuttings from stock plants grown at the highest levels of N, P, and K rooted less rapidly than those from medium and low levels. The stock plant nutrition treatments did not influence the growth of shoots in the explants cultured in vitro. | |
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