ISHS Acta Horticulturae 131: In Vitro Culture, XXI IHC
PROPAGATION OF PAPAYA THROUGH TISSUE CULTURE |
| Authors: | M.S. Rajeevan, R.M. Pandey |
| Abstract:
Tissue culture system using shoot tips from seedlings and lateral buds from female plants was tried for the propagation of papaya variety Coorg Honey Dew. Shoot tips and lateral buds could be established in Murashige and Skoog (MS) medium containing Naphthalene acetic acid (NAA) 0.5 – 10 μm and Kinetin (K) 25–50 μm. 6- Benzyl amino purine (BAP) 2 μm was optimal for multiplication of shoots. Indole butyric acid 10 μm was effective for inducing profuse rooting and normal plantlet formation. Better survival of plantlets was obtained in the potting mix, sand: soil: cowdung (1:1:1 v/v). Explants from plantlets viz., stem segments (B5 medium with NAA 10 μm and K 5 μm), root (B5 medium containing NAA and K each at 10 μm), petiole and leaf segments (MS medium with NAA 0.5 μm and BAP 2.5 μm) could give rise to callus in medium indicated in brackets. | |
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