ISHS Acta Horticulturae 131: In Vitro Culture, XXI IHC
ORGANOGENESIS AND PROPAGATION IN VITRO OF F1 HYBRID OF SOLANUM MELONGENA L. FROM VEGETATIVE SEGMENTS |
| Authors: | F. Macchia, F. Scaramuzzi, S. Porcelli |
| Abstract:
Excised leaf segments and buds of Solanum melongena F1 hybrid were cultured on Murashige and Skoog medium (MS) supplemented with various growth regulators. Leaf explants cultured on MS medium supplemented with IBA (indolebutyric acid) in the dark produced small callus and roots; when transferred to light developed adventive rooted shoots from the callus. Leaf explants cultured in the light on MS medium supplemented with IBA + Kn (6-furfurylamino-purine) produced green callus with numerous shoot primordia. The latter, transferred on the same medium additionated with IAA (indoleacetic-acid) or on MS medium with the lowest level of Kn (0.5 mg/l) and highest level of IBA (10 mg/l) produced more shoot primordia and rooted shoots. These were succesfully transferred to soil. Bud explants cultured on MS medium supplemented with IAA + Kn or IAA + BAP (6-benzylamino-purine) induced callus formation and growth of bud in 50–60% explants, while on MS with NAA (naphthalenacetic acid) + Kn or NAA + BAP induced only callus. The following root formation was obtained on MS with auxin (IBA) without cytokinins. Regenerated plantlets were transferred to soil where they flowered and set fruit. The results of tissue cultures of Solanum melongena F1 hybrid clearly demonstrated its potentiality to organogenesis "in vitro" and the possibility to obtain whole plant regeneration from excised leaf segments and axillary buds. | |
Download Adobe Acrobat Reader (free software to read PDF files) | |
