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ISHS Acta Horticulturae 130: XII International Symposium on Fruit Tree Virus Diseases

ADDITIONAL EVIDENCE ON SEED TRANSMISSION OF PLUM POX VIRUS IN APRICOT, PEACH AND PLUM PROVED BY ELISA

Authors:   M. Németh, M. Kölber
Abstract:
The purpose of our investigations was to detect plum pox virus in different parts of apricot, peach and plum seeds, to determine the rate of seed transmission, and to develop a rapid mass screening test for seed lots.

Depending upon species and cultivar, 21–93 % of the cotyledons, but only 0–11 % of the germs were found to be infected. Up to 91 % of the apricot seeds tested were found to be infected, and transmission to seedlings ranged from 0 to 14 %.

Group testing of seeds by ELISA is a suitable and sensitive mass screening method. Cotyledons of germinating seeds are the most reliable source of material. With this method, plum pox virus could consistently be detected when only one seed out of 20 was infected.

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