ISHS Acta Horticulturae 130: XII International Symposium on Fruit Tree Virus Diseases
SHOOT-TIP GRAFTING IN VITRO FOR ELIMINATION OF VIRUSES IN PEACH PLANTS (PRUNUS PERSICA BATSCH). |
| Authors: | L. Navarro, G. Llácer, M. Cambra, J.M. Arregui, J. Juárez |
| Abstract:
A method of shoot-tip grafting in vitro has been developed to obtain virus-free peach plants. It consists of aseptically grafting a 0.5 – 1.0 mm long shoot tip excised from a diseased plant on a 10-to-14-day-old Nemaguard seedling grown in vitro. Shoot tips were composed of the apical meristem and three to four leaf primordia. Between 45 and 70% of successful grafts were obtained and 60 to 70 % of the micrografted plants survived transplanting to soil. By this method peach plants free of several strains of prunus ringspot virus, prune dwarf virus and chlorotic leaf spot virus were obtained. | |
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