ROUTINE TESTING OF ENGLISH WALNUT MOTHER TREES AND GROUP TESTING OF SEEDS BY ELISA FOR DETECTION OF CHERRY LEAF ROLL VIRUS INFECTION

ISHS Acta Horticulturae 130: XII International Symposium on Fruit Tree Virus Diseases

ROUTINE TESTING OF ENGLISH WALNUT MOTHER TREES AND GROUP TESTING OF SEEDS BY ELISA FOR DETECTION OF CHERRY LEAF ROLL VIRUS INFECTION

Authors:

M. Kölber, M. Németh, P. Szentiványi

Abstract:
In Hungary the cherry leaf roll virus /CLRV-WH/ is widespread on English walnut. To select healthy mother trees and controlling seed lots used in nurseries the working out of a large-scale routine test was necessary. For this ELISA was the most suitable method.

In the course of working out of mass screening made by ELISA the following were stated:

the most suitable time to detect the virus is the period of bud break and the preceding one month,
the highest virus concentration can be found in the terminal buds,
the virus is not systemic in the tree, it is often localized only in certain buds, therefore on one tree at least 20 buds should be tested,
in large scale decetion of CLRV-WH group testing can be used applying batch samples of 20 buds,
in the virus concentration of various parts of stratified germinating seeds there is no significant difference,
during group testing of seeds the virus can be detected even if the grouped sample of 50 seeds contains only 1 infected seed.

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