ISHS Acta Horticulturae 130: XII International Symposium on Fruit Tree Virus Diseases
THE DETECTION OF PLUM POX VIRUS (PPV) BY INDICATOR PLANTS AND ENZYME-LINKED IMMUNOSORBENT ASSAY (ELISA) |
| Author: | G. Hamdorf |
| Abstract:
Plum pox virus (PPV) could be detected by ELISA in leaf samples from different varieties of plum, peach and apricot collected by advisors of the Plant Protection Service. Because of the sensitivity and reliability of ELISA for testing suspected samples this test can be used as a confirmatory test before valuable plants are removed in nurseries and orchards. However, it was not possible to detect PPV by ELISA in randomly selected leaf samples of 9–12-year-old originally virus-free mother trees, which had been reinfected by aphids. Only after the appearance of symptoms the virus could be detected in infected leaves. Furthermore, indexing of these trees by woody indicators (peach and apricot) generally failed because of the irregular distribution and low concentration of plum pox virus. | |
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