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ISHS Acta Horticulturae 1285: XXX International Horticultural Congress IHC2018: II International Symposium on Micropropagation and In Vitro Techniques

Growth and development of Hakonechloa macra in tissue culture depending on the type and color of light

Authors:   M. Parzymies, K. Rubinowska, D. Kozak, B.S. Ismael, A. Świstowska, B. Marcinek
Keywords:   Hakone grass, LEDs, fluorescent light, micropropagation, multiplication, rooting
DOI:   10.17660/ActaHortic.2020.1285.14
Abstract:
Hakonechloa macra (Hakone grass) is one of the most decorative ornamental grasses. It produces arching leaves forming cascading mounds. It is propagated through division, however, due to a slow growth it takes several years. Propagation of the species in vitro would allow to obtain a lot of plants in a relatively short time. The aim of the research was to study the effect of light color and type on growth and development of Hakonechloa macra in vitro. The plant material were 2-cm tufts derived from a stabilized tissue culture, which were placed on a Murashige and Skoog medium, supplemented with benzyladenine 3 mg dm‑3 and indolebutyric acid 0.3 mg dm‑3. The explants were cultivated at 16-h photoperiod, under fluorescent white light (W) or different color combinations of LEDs (1 blue with filter + 11 red, 1 green + 1 blue + 10 red, 2 green + 1 blue + 9 red, 1 blue + 11 red, 3 red + 1 blue + 7 white). The light intensity was 35 Ámol m‑2 s‑1. The obtained results were evaluated statistically. It was estimated that the light type and color influenced growth and development of Hakonechloa macra in vitro. There were no differences in the number of progeny tufts, however, the number of tufts explant‑1 was definitely lower under white fluorescent light or under LED light obtained from 1 blue with filter and 11 red diodes.

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