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ISHS Acta Horticulturae 1244: XII International Mango Symposium

Pollen conservation and in vitro germination studies in 'Appemidi' mango

Authors:   G.L. Veena, P.E. Rajashekaran, M.R. Dinesh, M. Sankaran
Keywords:   'Appemidi', pickle, conservation, pollen, in vitro, diversity
DOI:   10.17660/ActaHortic.2019.1244.6
Abstract:
Mango, the king of fruits, belonging to the family Anacardiaceae, is one of the most important fruit crops in the world. Several value added products are made from mango. One of those products, viz. pickle, is in great demand for export and for local markets. The Western Ghats in the peninsular region is largely unexplored for the immature whole fruited pickling cultivar, locally called ‘Appemidi’, for which there exists a large diversity. These pickles have a high potential for marketing and the cultivars need attention for conservation because these types are only grown in forest area and on the riverside. Hence, a study was planned to document the diversity and to conserve the genotypes by collecting pollen and scions. The self-incompatibility, caging technique in mango and embryological studies has shown the role of pollen in fertilization and development of zygote. The pollen grains of five ‘Appemidi’ genotypes were collected for in vitro study on pollen germination. The genotype ‘Gaddemar’ produced long pollen tube length (200.74 µm), a head length of 31.55 µm, but the germination percentage was comparatively less. The genotype ‘Naagara Appe’ recorded highest in vitro germination percentage of around 90%. Apart from germination media, temperature plays an important role in adequate pollen tube growth and successful fruit set. Study of in vitro germination and growth of pollen will help in establishing critical in vivo nutritional requirements for better fruit set and also for conservation of unique genotypes.

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