|Authors: ||N. Farinha, J.C. Gonšalves, M.A. Alves, T. Delgado, O. Anjos, C. Debiasi|
|Keywords: ||Vaccinium corymbosum, micropropagation, nodal segments, zeatin, 2iP, shoot regeneration, FTIR-ATR|
The Vaccinium genus has been studied by diverse research groups over the years, and micropropagation techniques have become a major tool in clonal multiplication on commercially cultivated and medicinally relevant Vaccinium species.
In this study one cultivar of Vaccinium corymbosum, 'Legacy', was used to evaluate the in vitro shoot initiation via shoot tip and nodal segments.
The first four segments of the plants were selected as explants, which were then individually cultured in tubes with modified WPM medium, supplemented with 7.89 ÁM 2iP. The shoot regeneration indices have shown significant differences between the four types of explants, and the fourth segment was found to be superior to the other tested segments, presenting 50% of shoot regeneration after 5 weeks of culture.
Moreover, the regenerated shoots from the fourth segment presented a high quality, both in aspect as well in the ability of developing new shoots.
The biochemical analyses, through FTIR-ATR, were used for the assessment of the substances content on the different segments used and the results have shown significant differences with different values of growth regulators obtained among them.
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