|Authors: ||T. Sala, A. Losa, L. Ferrari, P.E. Casali, B. Campion, M. Schiavi, G.L. Rotino|
|Keywords: ||Asparagus officinalis, Agrobacterium-mediated transformation, cut stem, androgenic embryoids calli|
Asparagus (Asparagus officinalis L.) is an economically important crop and it is cultivated for its spears.
This crop is a perennial and dioecious monocot.
Many are the agronomic traits of interest in the asparagus breeding.
We are interested to supplement our breeding research with genomic functional studies.
To this end, a reliable and efficient protocol for asparagus transgenic plant regeneration has to be set up.
Here we report the preliminary results of a research project aiming to optimize a transformation procedure in asparagus.
The possibility to transform and obtain transgenic plants, will also allow us to explore the potential of genome editing for basic and applied research in asparagus.
Two different explants were used to transform, cut stems and androgenic calli.
Two different Agrobacterium tumefaciens strains were tested (AGL1 and C58 GV2260); each one was co-cultivated singly with the two types of explants in different times of incubation (15, 25 and 60 min) for AGL1 and only 60 min for C58 GV2260. Preliminary data showed greater regeneration capacity of androgenic calli in comparison to the cut stems and this enabled us to obtain four putative transgenic shoots.
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