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ISHS Acta Horticulturae 1218: IX International Symposium on Kiwifruit

Conjugation in Pseudomonas syringae pv. actinidiae (Psa)

Authors:   M. Zhao, M. Butler, G. Taiaroa, R. Poulter
Keywords:   copper resistance, bacterial canker of kiwifruit, conjugation
DOI:   10.17660/ActaHortic.2018.1218.45
Abstract:
Copper-resistant field isolates of the kiwifruit pathogen Pseudomonas syringae pv. actinidiae biovar 3 (Psa3) have been identified in New Zealand. These isolates invariably carry large novel genetic elements, such as plasmids or integrative conjugative elements (ICEs), acquired by horizontal transfer from non-Psa3 species of bacteria already present in New Zealand. Here, we have determined whether these acquired elements are both necessary and sufficient to produce the observed copper-resistant phenotype. Methods were developed to transfer the ~100,000-bp ICEs and large (>100,000 bp) plasmids from the copper-resistant isolates to a sensitive recipient strain using natural conjugation. The ICEs or plasmids imparted the identical copper-resistance phenotype of the donor strains, showing that these elements were necessary and sufficient to confer copper resistance.

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