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ISHS Acta Horticulturae 1208: II Asian Horticultural Congress

Identification of walnut cultivars from Nikita Botanical Gardens using SSR-markers

Authors:   S. Khokhlov, E. Panyushkina, I. Balapanov, I. Suprun, S. Tokmakov
Keywords:   walnut, polymorphism, micro-satellite, molecular identification, genetic similarity
DOI:   10.17660/ActaHortic.2018.1208.7
Abstract:
Walnut (Juglans regia L.) is a crop with high potential for development in the South of Russia. In this study the identification of 15 walnut cultivars from Nikita Botanical Gardens (Yalta) was done by using seven SSR-markers developed for J. nigra. Cultivar 'Chandler' was taken as a standard. The allelic polymorphism data allowed to evaluate the genetic diversity of walnut on the territory of the peninsula. The number of alleles for each locus ranged from 6 to 14. A total of 69 alleles were obtained, on average 10 alleles per locus. The results of investigations allowed to determine the genetic similarity for studied cultivars and the standard. The dendrogram obtained after processing the experimental data divides the analyzed sample into 2 large clusters, which indicates the heterogeneity of the walnut nut population in the Crimea.

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