|Authors: ||I.C. Guţă, E.C. Buciumeanu, L.D. Tătaru, C.M. Topală|
|Keywords: ||virus-elimination, Vitis, ribavirin, oseltamivir, tissue culture|
Chemical drugs used in human medicine are shown to be effective for treating grapevine virus infections.
This is demonstrated under controlled conditions on in vitro cultures.
Virus-infected grapevine cultivars (Vitis vinifera L.) were grown for 30-40 days on agarized media supplemented with with 60 mg L-1 ribavirin and 120 mg L-1 oseltamivir viricides, used both individually and in admixture.
Regenerated plantlets were indexed by DAS-ELISA. Grapevine fanleaf virus could not be removed from 'Valerien' Grapevine leafroll associated virus 1-free plants 'Frāncuşă 15 Od.' were regenerated at a rate of 26 and 4% after exposure to ribavirin and combined viricides, respectively.
The treatment with combined viricides was effective and complete elimination of Grapevine fleck virus infection was observed.
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