ISHS Acta Horticulturae 1172: XIV EUCARPIA Symposium on Fruit Breeding and Genetics
Preliminary results on effectiveness of marker-assisted seedling selection applied to Mendelian traits in peach |
| Authors: | J.B. Mauroux, V. Diévart, C. Tuéro, L. Maillard, A. Maillard, M.J. Aranzana, I. Eduardo, I. Batlle, C. Cantin, J.C. Marcaillou, O.C. Mendoza, D. Bassi, I. Pacheco, S. Foschi, T. Pascal |
| Keywords: | DNA test, fruit, resistance, marker, polymorphism, SNPs-haplotype |
| DOI: | 10.17660/ActaHortic.2017.1172.80 |
| Abstract:
Under the 'FruitBreedomics' project (EU 7th Framework Program), 'pilot studies' were performed in peach to validate the marker-assisted seedling selection (MASS) applied to some Mendelian traits. Therefore, six breeding programs, either private, public or mixed, have phenotyped a total of 7,063 individuals belonging to 68 populations. These segregated either for fruit traits including non-glabrous/glabrous skin (G/g), white/yellow flesh (Y/y), flat/non-flat shape (S*/s*), sub-acid/acid flesh (D/d), and/or the resistance to the green peach aphid (Rm1/rm1). In parallel, leaf samples from each individual were collected and sent to an outsourced genotyping company, for both DNA extraction and genotyping with a set of SNP markers, developed within FruitBreedomics by linkage mapping and genome-wide association analysis, associated to the studied traits. Marker-assisted seedling selection (MASS) effectiveness was then checked by comparing predicted with observed phenotypes. Preliminary results globally show a 'high power' of the markers in predicting the phenotypes, with an average success of 95% for all traits. However, these findings need to be adjusted according to the DNA tests applicability, which varies depending on the available markers, the targeted trait (in particular the Y trait), as well as the parents (genetic backgrounds) used in the different breeding programs studied here. Further data and analyses are still needed to more efficiently use these results. | |
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