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ISHS Acta Horticulturae 1157: IX International Symposium on Grapevine Physiology and Biotechnology

Exogenous applications of nitric oxide affect the chlorophyll content and the expression of ELIPs-like protein in grapevine (Vitis vinifera L.)

Authors:   A. Riquelme, P. Alvarez, M. Pinto
Keywords:   'Sultanina', de-aetiolating, leaf development, chlorophyll accumulation, foliar proteins induction
DOI:   10.17660/ActaHortic.2017.1157.43
Nitric oxide (NO) has the ability to emulate dependent effects of light on plants. In particular, this molecule can inhibit the degradation of chlorophyll and promote the accumulation of this pigment in greening tissues. On the other hand, early light-induced proteins (ELIPs) are known to bind chlorophyll molecules, thus protecting them from degradation. NO, in turn, can also increase the expression of ELIPs. The aim of this study was to evaluate the effect of exogenous applications of nitric oxide on the chlorophyll content and on the expression of ELIPs-like proteins in greening young leaves of grapevines. Buds of grapevine 'Sultanina' were kept in the dark for two weeks to obtain aetiolated shoots. A nitric oxide treatment was obtained by spraying 100 ÁM S-nitroso-N-acetylpenicillamine solution onto shoots. Distilled water was sprayed onto control plants. The total chlorophyll content was measured by Arnon's method and ELIPs expression was detected by western blots, using pea-ELIPs antibodies. The results indicate that if the shoots were kept in dark after treatment, NO was unable to affect the chlorophyll content and the ELIP expression. However, if the greening young leaves were irradiated for two hours after NO treatment with 475 Ámol PAR m-2 s-1, NO was very effective for increasing the chlorophyll content and the ELIPs expression. This effect was greater as the light intensity increased.

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