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ISHS Acta Horticulturae 1156: VIII International Strawberry Symposium

The dynamic characteristics of soil enzyme activity and nematode diversity in replanted strawberry rhizosphere soil

Authors:   W.H. Li, Q.Z. Liu
Keywords:   continuous cropping, alkaline phosphate, urease, invertase, biodiversity, soil nutrient
DOI:   10.17660/ActaHortic.2017.1156.36
Abstract:
The eleven-year replanted strawberry rhizosphere soil in the greenhouse was sampled for studying the dynamic characteristics of soil physicochemical attributes, alkaline phosphatase, urease and invertase activity in different periods (Recover, Squaring, Young fruit, Full bearing),and soil nematodes diversity to explore the relationship with replanting. The soil physicochemical properties were determined by Kjeldahl nitrogen method (total N), atomic absorption method (K), Mo-Sb colorimetric method (P) and potassium dichromate method (Organic Matter). The soil enzymes were determined by colorimetric urea analysis method (urease activity), disodium phenyl phosphate (alkaline phosphatase), 3,5-dinitrosalicylic acid method (invertase). The soil nematodes were extracted by the method of sugar flotation and centrifugation. The results were shown as follows: the enzymes activities and nematode Shannon-wiener index (H') in replanted rhizosphere soil (RRS) were significantly lower than that in non-replanted rhizosphere soil (NRRS) in all periods. The urease and alkaline phosphatase reached to highest activity in flower bud formation period. The highest activity of invertase appeared in young fruit period. The preliminarily result indicated that replanting inhibited soil enzyme activity and reduced down nematode community diversity.

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