|Authors: ||D. Konavko, S. Malchev, J.F. Pothier, M. Jundzis, I. Moročko-Bičevska, F. Rezzonico|
|Keywords: ||bacterial diseases, sea buckthorn diseases, pathovars|
Pseudomonas syringae is an important bacterial pathogen causing significant damage to a wide variety of plant species worldwide.
Recently, P. syringae has become an increasing threat to Latvian fruit production.
Bacterial isolates were recovered from diseased fruit trees in Latvia with the aim of evaluating their genetic diversity, assess the range of pathogenic Pseudomonas species that are currently present in the country, and understand their potential threat for economically relevant plant species.
A collection of 207 Latvian isolates was preliminarily screened using a rapid fingerprinting approach based on Intact-Cell MALDI-TOF MS. Analysis of rpoD gene sequences then revealed an overall wide genetic diversity even among bacteria isolated from the same host.
Most isolates were found to cluster within the P. syringae species complex or in the Pseudomonas graminis subgroup.
Representative strains of these clades were selected for pathogenicity tests on different plant species to assess their actual host range.
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