ISHS


Acta
Horticulturae
Home


Login
Logout
Status


Help

ISHS Home

ISHS Contact

Consultation
statistics
index


Search
 
ISHS Acta Horticulturae 1147: IX International Symposium on Artichoke, Cardoon and Their Wild Relatives

Dormancy-related genes isolation in Cynara cardunculus var. sylvestris

Authors:   S.A. Raccuia, G. Puglia, H. Pappalardo, S. Argento, C. Leonardi, P. Calderaro, M.G. Melilli
Keywords:   Cynara cardunculus var. sylvestris, seed dormancy, Generalized Linear Models, gibberellins, GAox
DOI:   10.17660/ActaHortic.2016.1147.44
Abstract:
Seed physiological dormancy (PD) has been widely described as an inhibiting mechanism of germination completion of an intact viable seed under favourable conditions. Cynara cardunculus var. sylvestris germination is reported to be principally under control of temperature. Hereby we report the effect of fluctuating temperatures on C. cardunculus var. sylvestris germination behaviour with respect to corresponding constant regimes. Cypselae subjected to an alternating thermo-period of 15/5 or 25/15C germinated above 80%, while fruits imbibed at constant temperatures amounted to 40% for 10C and to 20% for 20C showing a significant decrease of germination (p<0.001). In PD seeds the regulation of dormancy is usually associated with the ABA-GA ratio and on ABA sensitivity. Therefore, we isolated GA2ox1 (Gibberellin 2-beta-dioxygenase 1), EXP (Expansin) and DOG1 (Delay Of Germination 1) genes in C. cardunculus, which were showed to have a key role in dormancy regulation in other plant species. The isolation of GA2ox1 gene was confirmed by DNA sequencing and protein domains analysis displayed the functional regions.

Download Adobe Acrobat Reader (free software to read PDF files)

1147_43     1147     1147_45

URL www.actahort.org      Hosted by KU Leuven      © ISHS