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ISHS Acta Horticulturae 1145: International Symposium on Biotechnology and Other Omics in Vegetable Science

Transformation of Cm-ADH gene to melon genotype

Authors:   E. Kocaman, Y. Yalcin Mendi, A. Latche, T. Izgu, I. El-Sharkawy
Keywords:   'Vedrantais', Agrobacterium tumefaciens, alcohol dehydrogenases, RNAi, regeneration
DOI:   10.17660/ActaHortic.2016.1145.22
Abstract:
Alcohol dehydrogenases (ADH) participate in the biosynthetic pathway of aroma volatiles in fruit by interconverting aldehydes to alcohols and providing substrates for the formation of esters. Two highly divergent ADH genes of cantaloupe Charentais melon (Cucumis melo var. cantalupensis 'Védrantais') have been isolated. This research is aimed at identifying the role of the Cm-ADH genes (Cm-ADH1 and 2) in the synthesis of aroma volatiles via the RNAi method. Different regeneration media were investigated to improve transformation efficiency. The best regeneration result was obtained from MS medium supplemented with 2.2 μM BAP and 0.54 μM NAA. Regeneration protocol has been applied to obtain transformed plants, using Agrobacterium tumefaciens which has a vector RNAi pFGC5941-(11406 bp) carrying genes of resistance to kanamycine and Bar. Transformed plants were characterized by PCR analysis.

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