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ISHS Acta Horticulturae 1143: VII International Symposium on Edible Alliaceae

Biological and molecular characterization of Garlic virus D and its effects on yields of garlic

Authors:   M.G. Celli, M.C. Perotto, D. Buraschi, V.C. Conci
Keywords:   garlic, Allium sativum L., filamentous virus, GarV-D, yield, genome
DOI:   10.17660/ActaHortic.2016.1143.28
Garlic is an agamic crop naturally infected by a viral complex including Potyvirus, Carlavirus and Allexivirus which reduces its yield. The current work aim was to characterize GarV-D biologically and molecularly and estimate its effect on garlic yields. Virion modal lengths were calculated by comparison of 136 viral particles and estimated in 734 nm. Total RNA from a sample was extracted and sent for sequencing (HiSeq 1500 Illumina). The contig comparison based on GenBank showed the presence of a 8,418 nucleotides (nt) sequence corresponding to a GarV-D isolate. The comparison of replicase and coat protein gen with other GarV-D isolates showed 83.4 to 93.3% and 86.5 to 89.1% nt identity respectively. Yield of virus-free garlic, garlic only infected with GarV-D and plants infected with a viral mixture was evaluated in assays under anti aphid cages and in field. At the end of the growth cycle, significant differences in weight and cloves number of garlic bulbs cultivated in field were observed. The garlic infected with GarV-D isolate showed a reduction of 12.3% of weight, 6.7% of bulb caliber and 40.2% of number of cloves when compared with healthy garlics. Some plants of the virus-free and GarV-D isolated treatments maintained in the field showed infections with other viruses (OYDV, LYSV) which could have affected their yield. This situation may indicate that when GarV-D is isolated, no yield damage is produced, however when combined with others (as it happened with plants in field) synergic effect occurs and damage is more significant.

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