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ISHS Acta Horticulturae 1143: VII International Symposium on Edible Alliaceae

Determination of alliin and allicin in the plant of Allium scorodoprasum L. subsp. rotundum by using the infrared spectroscopy technique

Authors:   B. Tasci, H. Kutuk, I. Koca
Keywords:   allicin, alliin, Allium, IR
DOI:   10.17660/ActaHortic.2016.1143.19
Abstract:
In this study, alliin and allicin have been investigated in the leaf and bulb parts of Allium scorodoprosum L. subsp. rotundum by the Fourier transform infrared (FTIR) spectroscopy. The plant samples were obtained from three different locations in Amasya where naturally grows. Different spectra have been obtained from the plantRSQUOs leaf and bulb parts. The obtained peaks and their groups can be assigned like this; in the spectrum of the bulb part of the plant; 1st peak (3285.35 cm-1) belongs to (CH=CH2) group of allyl, 2nd peak (2950.9 cm-1) belongs to group of aliphatic CH, 3rd peak (1638.15 cm-1) belongs to C=C, 4th peak (1032 cm-1) belongs to S=O, 6th peak (891.88 cm-1) belongs to C-S, and 7th peak (707.45 cm-1) belongs to S-S single bond. In the spectrum of the leaf part of the plant,1st peak (3372.57 cm-1) belongs to OH group in the group of (COOH), 2nd peak (2916.72-2848.93 cm-1, doublet) belongs to the group of NH2, 3rd peak (1734.36 cm-1) belongs to C=O group in the group of (COOH), 4th peak (1638.04 cm-1) belongs to C=C double bond in the group of allyl, 5th peak (1172.24 
cm-1) belongs to C-O single bond, 6th peak (1100.5 cm -1) belongs to C-N, 7th peak (1011.15 cm-1) belongs to S=O bond, and 8th peak (958.4 cm-1) belongs to C-S single bond. When the peak giving parts of the functional groups in the structural formulas of allicin and alliin are analysed, the existence of both compounds are observed as a result of the spectral measurement. In the leaf part of plant, alliin was the main component because of the NH2 double peaks while in the bulb part of plant, allicin was the main component.

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