|Authors: ||Prakash Babu Adhikari, Chul-Soo Yoon, Won-Hee Kang|
|Keywords: ||tomato, anther, shoot regeneration, 'Campari', buds|
The efficiency of callogenesis and subsequent shoot regeneration using different lengths of flower buds of tomato 'Campari' and 'Temptation' was analyzed in this study.
Calli were induced on MS media supplemented with different combinations of growth regulators [2 mg L-1 indole-3-acetic acid (IAA) plus 1 mg L-1 N6-(2-isopentenyl) adenine (2ip), 2 mg L-1 IAA plus 3 mg L-1 6-benzylaminopurine (BA), and 2 mg L-1 IAA plus 0.25 mg L-1 zeatin riboside (ZR)]. The developed calli were transferred to the MS medium containing 2 mg L-1 IAA plus 1 mg L-1 2ip for organogenesis.
The developed shoot buds were transferred to the medium containing 2 mg L-1 IAA plus 0.25 mg L-1 ZR for shoot regeneration.
The flower bud length ranging from 3 mm to >6 mm for 'Campari' and 4 mm to >7 mm for 'Temptation' produced calli.
For 'Campari', flower buds of 4 mm gave the highest number of calli and shoots (63 and 29% respectively). However, for 'Temptation', there were flower buds of 7 mm which produced more calli and shoots (72 and 17% respectively). The bud length was found to be strongly negatively correlated with callogenesis and also negatively correlated with shoot regeneration.
For both cultivars, MS supplemented with 2 mg L-1 IAA plus 1 mg L-1 2ip gave highest number of callogenesis and shoot regeneration (71 and 29% respectively for 'Campari' and 31 and 9% respectively for 'Temptation'). This finding would be an efficient technique for callogenesis and shoot regeneration in tomato anther culture.
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