ISHS


Acta
Horticulturae
Home


Login
Logout
Status


Help

ISHS Home

ISHS Contact

Consultation
statistics
index


Search
 
ISHS Acta Horticulturae 1133: XI International Rubus and Ribes Symposium

Natural microbiota of raspberries (Rubus idaeus) and strawberries (Fragaria ananassa): microbial survey, bacterial isolation and identification, and biofilm characterization

Authors:   J.L. Baugher, L.A. Jaykus
Keywords:   raspberry (Rubus idaeus var. strigosus 'Heritage'), Strawberry (Fragaria ananassa ‘Chandler’), Norovirus, microbiota, biofilm
DOI:   10.17660/ActaHortic.2016.1133.82
Abstract:
Human norovirus (HuNoV) outbreaks have been associated with the consumption of contaminated fresh raspberries (Rubus idaeus) and strawberries (Fragaria ananassa). The dynamics of HuNoV binding to berries are unknown: viruses may attach to berry-specific ligands; bind non-specifically (electrostatic and hydrophobic interactions); or bind in a facilitated manner such as in association with native berry microflora. In fact, HuNoV have been shown to bind to specific structures found on the extracellular polymeric substances (EPS) produced by biofilm-producing bacteria. A microbial survey was done on (Rubus idaeus var. strigosus 'Heritage') raspberries and 'Chandler' strawberries obtained from local U-Pick farms (Angier, NC and Raleigh, NC, respectively) by plating them on selective and differential media. A total of 172 (n=98 from raspberries; n=74 from strawberries) unique bacterial isolates were identified, with the microbial profiles differing by berry type. Selected isolates were tested for the capacity to form biofilms using a crystal violet-based assay. Biofilm production varied greatly among isolates, but strains of Pantoea agglomerans, Pseudomonas moraviensis, Klebsiella oxytoca, and Enterobacteriaceae spp. were the strongest biofilm producers (A405>2.2). These strong biofilm-producing strains will be used in future studies to characterize the attachment of HuNoV to the natural berry microflora and determine if adherence to specific microflora components (e.g., EPS) significantly contributes to the attachment and persistence of HuNoV on berries.

Download Adobe Acrobat Reader (free software to read PDF files)

1133_81     1133     1133_83

URL www.actahort.org      Hosted by KU Leuven      © ISHS