|Authors: ||G. Bufler, Y. Mor|
The method of Lizada and Yang (1979) for the extraction and assay of 1-aminocyclopropane-1-carboxylic acid (ACC) was adapted for use with carnations (Dianthus caryophyllus L.). Flower tissue was extracted with either 3% HC104 or 80% ethanol.
The presence of ethanol in the assay mixture resulted in production of ethylene not derived from ACC (at room temperature), and it was therefore necessary to remove all traces of ethanol by evaporating the extract.
This concentration step increased the sensitivity of the ACC assay.
Another non-ACC source of ethylene in the ACC assay was observed in both HC104 and ethanol extracts from senescing carnation flowers treated previously with 2 mM -aminoxyacetic acid (AOA), an inhibitor of ACC synthase.
The nature of this artifact is discussed.
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