ISHS Acta Horticulturae 112: Symposium on Breeding and Machine Harvesting of Rubus
IN VITRO CULTURE OF THREE RUBUS SPECIES |
| Authors: | D.J. Donnelly, R. Stace-Smith, F.C. Mellor |
| Abstract:
Meristem tip culture followed by in vitro micropropagation is a valuable technique for mass producing disease-free germplasm. Meristem tip development, micropropagation and rooting of black raspberry (Rubus occidentalis L.) cv. Munger were successfully carried out in Murashige-Skoog inorganic salts with 30 g/L sucrose, 0.5 mg/L pyridoxine-HCI, 0.5 mg/L nicotinic acid, 0.4 mg/L thiamine-HCI, 2.0 mg/L glycine, 100 mg/L inositol, 10 mg/L cysteine-HCI, 0.1 mg/ L 6-benzylaminopurine (BAP) and 1.0 mg/L indole butyric acid (IBA). Meristem tips from thornless loganberry (R. loganobaccus Bailey) and nine red raspberry selections (R. idaeus L.) had a higher survival rate when excised into medium without cysteine-HCL or hormones, and then subcultured after 1 mo into a medium with 1.0 mg/L BAP, and 0.1 mg/L IBA. When meristem tips were started in medium with hormones the rate of survival decreased, but the growth rate of the survivors increased. The success of micropropagation depended on the carbon source and its concentration, the pH and the concentration of BAP in the medium. Success varied among red raspberry selections. Rooting of micropropagated shoot cuttings was best in medium with 0.5–0.7 mg/L IBA, which also promoted considerable top growth. The effect of pre-culture treatment was investigated: the use of etiolated red raspberry source plants was not advantageous; incubating source plants at 37°C for up to 8 wk improved survival and accelerated rooting of black raspberry meristem tips, increased red raspberry meristem tip survival slightly and did not affect survival of loganberry meristem tips. Rooted shoots survived planting directly into soil, but could be stored in vitro at 10°C for up to 3 mo before planting without deleterious affects. | |
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