ISHS Acta Horticulturae 110: V International Symposium on Virus diseases of Ornamental Plants
A POTEXVIRUS INFECTING NANDINA DOMESTICA 'HARBOR DWARF' |
| Authors: | F.W. Zettler, E. Hiebert, E. Maciel-Zambolim, M.M. Abo El-Nil, R.G. Christie |
| Abstract:
A potexvirus isolated from Nandina domestica 'Harbor Dwarf' is tentatively named nandina mosaic virus (NMV). Manually inoculated seedlings of Nan. domestica expressed systemic mosaic symptoms without foliar distortion; these symptoms were evident on the first or second leaves produced after inoculation but were expressed intermittently thereafter. The virus infected Nicotiana benthamiana and Nicotiana megalosiphon systemically. Chenopodium amaranticolor and Gomphrena globosa were local lesion hosts. The modal lengths of virus particles from Nan. domestica, N. benthamiana, and N. megalosiphon leaf extracts were 448, 438, and 471 nm, respectively. When aphids (Myzus persicae) were allowed ≤1 min acquisition/access periods on NMV-infected N. benthamiana leaves and subsequently transferred to healthy N. benthamiana, they failed to transmit NMV. Fibrous cytoplasmic inclusions consisting of aggregated virus particles were seen by light and electron microscopy. The virus was purified from N. benthamiana by clarification in n-butanol and chloroform, two precipitations using 8% (final concn) polyethylene glycol 6000, and differential centrifugation. A yield of 0.65 mg of NMV per gram of host tissue was thus obtained. The 260/280 ratio of the purified preparation was 1.37. The NMV capsid protein molecular weight was ca. 20,500 daltons. Antiserum to NMV was obtained from rabbits by a series of 4 weekly intramuscular injections, each consisting of 2.4 mg of virus. In immunodiffusion tests, the antiserum had a titer of ¼ against purified virus. In reciprocal tests, serological reactions were not noted between NMV and 6 other potexviruses. In a unilateral test, NMV reacted identically with a purified preparation of a previously described potexvirus of nandina. | |
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