ISHS Acta Horticulturae 110: V International Symposium on Virus diseases of Ornamental Plants
SOME HOSTS AND PROPERTIES OF AN ISOLATE OF NERINE VIRUS X FROM AGAPANTHUS PRAECOX SUBSP. ORIENTALIS |
| Authors: | S. Phillips, A.A. Brunt |
| Abstract:
A virus detected in Agapanthus praeco x subsp. orientalis (Liliaceae) in S.E. England is serologically indistinguishable from nerine virus X (NVX) isolated previously from Nerine sarniensis (Amaryllidaceae) in the Netherlands. Isolates of NVX from A. orientalis, unlike those from N. sarniensis, are readily sap-transmissible, easily maintained in test plants and purified without difficulty. A typical isolate from A. orientalis infected 14 of 44 species from 8 of 12 families. It induced systemic infection in A. orientalis, N. sarniensis and Chenopodium quinoa, but local infections only in 11 other susceptible herbaceous species of the Chenopodiaceae, Amaranthaceae, Aizoaceae, Solanaceae, Compositae and Papilionaceae. The virus is very stable in vitro: sap from C. quinoa was still infective after dilution to 1/1,000,000, after 10 min at 95–100°C and for 1 yr at 20°C. It is readily transmissible by sap-inoculation, but it was not seed-borne in C. quinoa. The virus has flexuous filamentous particles mostly measuring c. 11 x 525–550 nm. The particles sediment as a single component with a sedimentation coefficient of 120 S and have a buoyant density in caesium chloride of 1.31 g/cm3. They contain c. 5% single-stranded RNA of 2.5 million mol. wt and a single polypeptide of 25,600. NVX is serologically distantly related to potato X, viola mottle, hydrangea ringspot and commelina X viruses, but it is serologically unrelated to 9 other distinct potexviruses. | |
Download Adobe Acrobat Reader (free software to read PDF files) | |
