ISHS Acta Horticulturae 110: V International Symposium on Virus diseases of Ornamental Plants
CHRYSANTHEMUM STUNT AND CHLOROTIC MOTTLE. ESTABLISHMENT OF HEALTHY CHRYSANTHEMUM PLANTS AND STORAGE AT LOW TEMPERATURE OF CHRYSANTHEMUM, CARNATION, CAMPANULA AND PELARGONIUM IN TUBES |
| Author: | N. Paludan |
| Keywords: | Chrysanthemum, viroids, inactivation, ornamentals, cool storage, hypobaric storage |
| Abstract:
Chrysanthemum stunt virus (CSV) is widespread wherever chrysanthemum cultures are grown, causing severe stunt and deterioration in quality. Chrysanthemum chlorotic mottle virus (ChCMV) which causes slight reduction in growth, occurs only to a limited extent. The establishment of healthy plants has been carried out with divergent results and in many cases without success. A safer method of producing healthy chrysanthemum plants has been carried out by heat treatment and meristem-tip cultures. Furthermore, storing chrysanthemum, carnation, campanula and pelargonium plants in tubes at low temperatures and in some cases at hypobaric conditions has been attempted. CSV has been inactivated increasingly with the decreasing size of the meristem-tip in connection with disinfection of the scalpel, making it red-hot between the individual meristem-tips. Meristem-tips from the terminal buds have produced the greatest number of CSV-free plants. Heat treatment of either the mother plants or the meristem-tip plants in tubes had no certain influence, nor had the addition of auxin (2, 4 D) to the nutrient solution. ChCMV has only been inactivated in one single plant by similar treatments. Storage of plants in tubes at low temperature has been possible, comprising carnation and chrysanthemum during one year, campanula and pelargonium temporarily for eight and six months. The stored plant material has proved still suitable for cutting production after further culturing at optimal conditions. Hypobaric storage has not been to any advantage either for carnation or chrysanthemum. | |
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