ISHS Acta Horticulturae 110: V International Symposium on Virus diseases of Ornamental Plants
PRODUCTION OF VIRUS-FREE ORNAMENTAL PLANTS IN TISSUE CULTURE |
| Authors: | T.C. Allen, W.C. Anderson |
| Abstract:
Lilies, bulbous irises, rhododendrons and hydrangeas are being produced in tissue culture at the Northwestern Washington Research and Extension Unit in Washington, then assayed for viruses at Oregon State University. Most plants are assayed three times, in the one to two leaf stage, after established and growing in soil and when starting to flower. Plants free of viruses in all three assays have not been found to contain viruses in additional assays. Lilium longiflorum cvs. "Ace" and "Nellie White", were freed of lily symptomless virus after shoot tip apex culture. Virus-free bulbous irises were obtained by excising and culturing small bulblets that form on the virus-infected mother bulb basal plate. Rhododendrons and hydrangeas obtained after shoot tip apex culture are still in initial stages of assay for viruses. Virus detection methods used were assay host symptoms, electron microscopy, enzyme-linked immunosorbent assay and immunosorbent electron microscopy. The latter technique was preferred. | |
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